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Mumps
Lab Criteria for Diagnosis
- Isolation of mumps virus from a clinical specimen (buccal swab or urine).
- Detection of viral RNA by reverse transcription polymerase chain reaction
(RT-PCR)
- Note: The interpretation of a positive RT- PCR result without demonstration of mumps
growth in tissue culture must be interpreted carefully, particularly among
persons whose symptoms do not meet the clinical definition of mumps.
- A four-fold rise between acute- and convalescent-phase titers in serum
mumps immunoglobulin G (IgG) antibody level by standard serologic assay.
- Note: This rise may be undetected by this method in infected
individuals who have previously been vaccinated against mumps.
- Positive serological test for mumps IgM antibody.
Specimens to Collect
- Acute and convalescent sera
- Collect acute serum ASAP after parotitis onset and no later than 7 days post parotitis onset.
- Collect convalescent serum >= 3 weeks after the acute serum was collected.
- Buccal swab
- For the mumps viral specimen a parotid gland/buccal swab is the preferred specimen.
- Collect buccal swabs no later than 9 days post parotitis onset.
- Massage the parotid
gland area (the space between the cheek and teeth just below the ear) for about 30 seconds
prior to collection of the buccal secretions. The parotid duct (Stensen's duct) drains in this space near the
upper rear molars. A throat swab (oropharyngeal or nasopharyngeal swab) can also be collected and added together
with the buccal swab.
- Place the swab in a tube containing 2-3 mls of viral transport medium (i.e., Hank's
Buffered Saline Solution) or other sterile isotonic solution (phosphate buffered saline or cell
culture medium).
- Urine
- Collect 5-10 mls from clean catch urine and store in a screw top sterile
container, preferably a 15 ml centrifuge tube.
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